A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus

نویسندگان

  • Chun-Hsien Tseng
  • Hsiang-Jung Tsai
  • Chung-Ming Chang
چکیده

INTRODUCTION The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. METHOD This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. RESULT AND CONCLUSION This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.

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عنوان ژورنال:

دوره 2014  شماره 

صفحات  -

تاریخ انتشار 2014